Expression of voltage gated sodium channel Nav1.9 in experimental pulpal lesions in the rats
10.3760/cma.j.issn.1002-0098.2014.02.007
- VernacularTitle:电压门控钠离子通道Nav l.9在牙髓炎牙髓中表达的动物实验
- Author:
Le XU
1
;
Xinwei ZHU
;
Qingfeng CHEN
;
Yuanping HU
;
Ling ZHU
;
Yong JIANG
Author Information
1. 安徽医科大学口腔医学院·口腔医院口腔预防教研室安徽省口腔疾病研究中心实验室
- Keywords:
Pulpitis;
Animals,laboratory;
Toothache;
Sodium channels,voltage-gated
- From:
Chinese Journal of Stomatology
2014;49(2):95-100
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between pulpitis pain and voltage-gated sodium channel(Nav 1.9) by detecting the expression of Nav 1.9 at different time points of the rat pulpal lesion model.Methods Thirty-six SD pulpal lesions rat models were divided into three experimental groups,1 d (n =12),3 d (n =12) and 5 d group(n =12).Normal SD rats served as control(n =12).Tumor necrosis factor-α(TNF-α) and Nav 1.9 mRNA expressions were evaluated by reverse transcription PCR(RT-PCR).Nav1.9 protein expressions were analyzed by enzyme-linked immunosorbent assay (ELISA) and Western blotting.Results The expression of TNF-α in the experimental group (1 d:0.514 ± 0.098,3 d:0.739 ±0.104,5 d:1.238 ± 0.082) was higher than those in the control group (0.147 ± 0.016) (P ＜0.01).Navl.9 mRNA was up-regulated markedly in experimental groups (1 d:0.296 ± 0.038,3 d:0.409 ±0.013,5 d:0.487 ±0.028),compare with control group(0.223 ±0.020) (P ＜0.05).The ELISA results revealed that the level of Nav 1.9 in control pulp tissue was (4.013 ±0.292) μg/L,in painful pulp tissue of 1 d group was (5.143 ± 0.101) μg/L,in painful pulp tissue of 3 d group was (5.835 ±0.088) μg/L and in painful pulp tissue of 5 d group was (6.307 ± 0.137) μg/L (P ＜ 0.05).Western blotting showed the expression of Navl.9 in experimental groups(1 d:0.106 ±0.007,3 d:0.170 ±0.013,5 d:0.238 ± 0.004) was up-regulated significantly compared with control group (0.073 ± 0.004)(P ＜ 0.05).Conclusions The level of Nav 1.9 had a significant inerease in painful pulp tissue.Moreover,with the degree of pain aggravation,the expression of Nav.l.9 increased in pulp tissue.It suggests that Nav 1.9 may play an important role in the development of pulpitis pain.
