Anti-tumor effect of chemotherapeutic drugs on human gastric cancer cells in vitro and the relationship with Bcl-2 expression.
- Author:
Ming GENG
1
;
Ying-Chun YIN
;
Yong-Cheng CAO
;
Zhi-Jie FU
;
Yan-Hong TAI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Antineoplastic Agents; pharmacology; therapeutic use; Cell Survival; Cisplatin; pharmacology; therapeutic use; Doxorubicin; pharmacology; therapeutic use; Drug Screening Assays, Antitumor; Female; Fluorouracil; pharmacology; therapeutic use; Humans; Male; Middle Aged; Mitomycin; pharmacology; therapeutic use; Mitomycins; pharmacology; therapeutic use; Paclitaxel; pharmacology; therapeutic use; Proto-Oncogene Proteins c-bcl-2; metabolism; Stomach Neoplasms; drug therapy; metabolism; pathology; Tumor Cells, Cultured
- From: Chinese Journal of Gastrointestinal Surgery 2008;11(3):276-279
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate in vitro anti-tumor effect of chemotherapeutic drugs on human gastric cancer cells, and investigate the relationship with Bcl-2 expression.
METHODSSingle cell suspension was prepared from fresh gastric cancer tissue and exposed to taxol (Tax), 5-fluorouracil (5-FU), cisplatin (CDDP), adriamycin (ADM), mitomycin (MMC) respectively for 48 hours. Metabolic activity and inhibitory rate of cells were detected by MTT assay. Expression of Bcl-2 was examined with immunohistochemistry.
RESULTSThe inhibitory rates of cancer cells exposed to chemotherapeutic drugs were different and Tax, 5-FU, CDDP had remarkably higher rates than ADM and MMC. The lower differentiated gastric cancer cells were more sensitive than the higher ones. Positive expression rate of Bcl-2 was 80% and the positive cells showed resistance to 5-FU, ADM and MMC.
CONCLUSIONSChemosensitive testing by MTT assay can constitute the prediction for the application of chemotherapeutic drugs individually. Overexpression of Bcl-2 may contribute to multiple drug-resistance of tumors.
