Identification of Haemaphysalis longicomis and Rhipicephalus microplus

VernacularTitle:长角血蜱和微小扇头蜱的形态与分子生物学鉴定
Author: Xin-Cheng QIN ¹ ; Jun-Hua TIAN ; Jian-Bo WANG ; Xin LU ; Qiang-Zheng SUN ; Dong JIN ; Dun-Jin ZHOU ; Jian-Guo XU ; Yong-Zhen ZHANG
Author Information

1. 中国疾病预防控制中心传染病预防控制所
Keywords: Haemaphysalis longicomis; Rhipicephalus microplus; Identification
From: Chinese Journal of Epidemiology 2011;32(6):608-612
CountryChina
Language:Chinese
Abstract: Objective To establish a method combined morphology and molecular marker for identifying Haemaphysalis longicomis and Rhipicephalus microplus. Methods Ticks were collected from domestic animals and wild environment in epidemic area of Hubei and Henan provinces where cases of fever with thrombocytopenia syndrome were prevalent. We classified the ticks by morphology characteristics before 12S rDNA of ticks were amplified by PCR and subsequently sequenced. Phylogenetic tree was constructed by PAUP4.0. Results The ticks belonged to Haemaphysalis longicomis and Rhipicephalus microplus through observation and analysed by the morphological characteristics of the ticks. 12S rDNA was cloned and sequenced while data confirmed the morphological identification of the results. Conclusion The method based on morphology that combined with molecular marker seemed a good method for the identificaton of ticks.