Curative machanism of Shenle capsule on 5/6 nephrectomy rats.
- Author:
Ri-Bao WEI
1
;
Wu-Xing ZHANG
;
Xiang-Mei CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Capsules; Codonopsis; chemistry; Drug Combinations; Drugs, Chinese Herbal; isolation & purification; pharmacology; Female; Kidney; metabolism; Kidney Failure, Chronic; drug therapy; metabolism; Leeches; chemistry; Male; Materia Medica; isolation & purification; pharmacology; Nephrectomy; Plasminogen Inactivators; metabolism; Polyporales; chemistry; RNA, Messenger; biosynthesis; Rats; Rats, Sprague-Dawley; Tissue Plasminogen Activator; metabolism; Transforming Growth Factor beta; biosynthesis; genetics; Urokinase-Type Plasminogen Activator; metabolism
- From: China Journal of Chinese Materia Medica 2004;29(8):770-773
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore curative machanism of Shenle capsule on the 5/6 nephrectomy rats.
METHODFibrin plate method was applied to examine activity of urinary plasminogen activator(PA). Semi-quantitative analysis was used to observe stained intensity and area of tissue-type plasminogen activator, urokinas-type plasminogen activator/ plasminogen activator inhibitor(tPA, uPA/PAI-1)in remnant renal tissue. Northern blot was employed to analyze the expression of transforming growth factor (TGF-beta) mRNA.
RESULTIn model control group, the urinary PA activity and protein expression of tPA, uPA were down-regulated, but protein expression of PAI-1, TGF-beta mRNA was up-regulated in remnant renal tissue. In each treated group, the urinary PA activity and protein expression of tPA/uPA were enhanced,but the protein expression of PAI-1, TGF-beta mRNA decreased simultaneously.
CONCLUSIONShenle capsule can delay glomerulosclersis and tubulointerstitial fibrotic lesions of remnant kidney by improving the activity of urinary PA and modulating the expression of tPA, uPA/PAI-1 and TGF-beta mRNA.
