Effect of Human Umbilical Cord Mesenchymal Stem Cells on Etoposide-induced Nalm-6 Cell Apoptosis.
- Author:
Jian-Ling WANG
1
;
Dong LI
1
;
Xue LI
1
;
Pan-Pan ZHOU
1
;
Xiu-Li JU
2
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Caspase 3; metabolism; Cell Cycle; Cell Line, Tumor; Cells, Cultured; Coculture Techniques; Etoposide; adverse effects; Flow Cytometry; Humans; Mesenchymal Stromal Cells; cytology; Proto-Oncogene Proteins c-bcl-2; metabolism; Umbilical Cord; cytology; bcl-2-Associated X Protein; metabolism
- From: Journal of Experimental Hematology 2016;24(1):178-183
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of human umbilical cord mesenchymal stem cells (hUC-MSC) on VP16-induced apoptosis of Nalm-6 cells.
METHODShUC-MSC were isolated and identified using morphological observation and flow cytometry, then Nalm-6 cells were treated with hUC-MSC with or without VP16. Apoptosis and cell cycle were assayed by FACS. The mRNA levels of apoptosis-related genes BCL-2, BAX and caspase-3 were detected by quantitative RT-PCR, and the protein levels of BCL-2, BAX and caspase-3 were examined by Western blot.
RESULTSFACS showed that hUC-MSC inhibited the proliferation and decreased apoptosis of Nalm-6 cells resulted from VP16. The quantitative RT-PCR showed that hUC-MSC increased the mRNA expression level of BCL-2 and decreased the expression level of BAX and caspase-3 (P < 0.05). Western blot showed that the protein expression level of BCL-2 increased, and expression level of BAX and caspase-3 decreased in Nalm-6 cells after co-culture with hUC-MSC (P < 0.05).
CONCLUSIONhUC-MSC may protect Nalm-6 cells from apoptosis induced by VP16 through regulation of BCL-2, BAX and caspase-3.
