Inhibitory effect of iguratimod on TNFalpha production and NF-kappaB activity in LPS-stimulated rat alveolar macrophage cell line.
- Author:
Ye JIANG
1
;
Wei LÜ
;
Shu-Qin YU
;
Lin YAO
;
Guang-Lin XU
;
Xi-Ran ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anti-Inflammatory Agents, Non-Steroidal; pharmacology; Cell Line; Cell Proliferation; drug effects; Chromones; pharmacology; Lipopolysaccharides; Macrophages, Alveolar; drug effects; metabolism; NF-kappa B; metabolism; RNA, Messenger; biosynthesis; genetics; Rats; Sulfonamides; pharmacology; Tumor Necrosis Factor-alpha; biosynthesis; genetics
- From: Acta Pharmaceutica Sinica 2006;41(5):401-405
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effect of iguratimod (T-614), a non-steroidal anti-inflammatory drug, on TNFalpha mRNA expression and TNFalpha production, and on the activity of nuclear factor-kappaB (NF-kappaB) in the rat alveolar macrophage cell line (NR8383) activated by LPS.
METHODSNR8383 cells were pretreated with T-614 (13.4, 26.7, 53.4 micromol x L(-1)), then were stimulated with LPS. The production of TNFalpha in the supernatant of NR8383 was assayed by enzyme-linked immunosorbent assay (ELISA). The TNFalpha mRNA level was determined by a semi-quantitative PCR assay. Assessment of the NF-kappaB DNA binding activity was performed by an ELISA kit.
RESULTST-614 inhibited LPS-stimulated mRNA expression and production of TNFalpha in a concentration-dependent manner, as well as the activity of NF-kappaB. The IC50 value of effect of T-614 on TNFalpha level was 26.2 micromol x L(-1).
CONCLUSIONThe inhibitory effect of T-614 on the production of TNFalpha in LPS-stimulated NR8383 cells may be mediated by suppression of NF-kappaB activity.
