S phase cell percentage normalized BrdU incorporation rate, a new parameter for determining S arrest.

Peng ZHAO; Juan Ling FU; Bi Yun YAO; Yong Rui JIA; Zong Can ZHOU

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S phase cell percentage normalized BrdU incorporation rate, a new parameter for determining S arrest.

Author: Peng ZHAO ¹ ; Juan Ling FU ² ; Bi Yun YAO ³ ; Yong Rui JIA ⁴ ; Zong Can ZHOU ²
Author Information

1. Department of Toxicology, Peking University Health Science Center, Beijing 100191, China; Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Peking University Health Science Center, Beijing 100191, China.
2. Department of Toxicology, Peking University Health Science Center, Beijing 100191, China.
3. Department of Toxicology, Peking University Health Science Center, Beijing 100191, China
4. Medical and Healthy Analytical Center, Peking University Health Science Center, Beijing 100191, China.
Publication Type:Journal Article
MeSH: Bromodeoxyuridine; pharmacokinetics; Cell Proliferation; DNA Damage; Epithelial Cells; cytology; Humans; S Phase; S Phase Cell Cycle Checkpoints
From: Biomedical and Environmental Sciences 2014;27(3):215-219
CountryChina
Language:English
Abstract: In this study, a new parameter, S phase cell percentage (S fraction) normalized BrdU (SFN-BrdU) incorporation rate, was introduced to detect S arrest. The results showed a positive linear correlation between the BrdU incorporation rate and the S fraction in unperturbed 16HBE cells. Theoretical analysis indicated that only S arrest could result in a decrease in the SFN-BrdU incorporation rate. Additionally, the decrease in SFN-BrdU incorporation rate and the activation of DNA damage checkpoints further demonstrated that S arrest was induced by diethyl sulfate treatment of 16HBE cells. In conclusion, SFN-BrdU incorporation rate can be used to detecting S arrest.