Cloning and sequence analysis of MYB transcriptional regulator SmP gene of Saussurea medusa Maxim.
- Author:
Zhi-Ping JIN
1
;
De-Xiu ZHAO
;
Chuan-Ling QIAO
;
Wen-Quan QU
;
Ya-Qiong CHEN
;
Chun-Xiang FU
Author Information
1. Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Cloning, Molecular;
Molecular Sequence Data;
Phylogeny;
Plant Proteins;
chemistry;
classification;
genetics;
metabolism;
Polymerase Chain Reaction;
Saussurea;
classification;
genetics;
metabolism;
Sequence Homology, Amino Acid;
Transcription Factors;
chemistry;
genetics;
metabolism
- From:
Chinese Journal of Biotechnology
2003;19(3):368-371
- CountryChina
- Language:Chinese
-
Abstract:
A full-length cDNA encoding a MYB-related regulatory gene was isolated from a cDNA library prepared from mRNAs of the red line callus of S. medusa by TD-PCR. The cDNA, designated SmP, is 969 nucleotides long and has an open reading frame of 771 bp with a deduced amino acid sequence of 256 residues. The putative protein of SmP has two typical conversed R2R3-Myb DNA-binding domains in N-terminal and displays a rather high degree of similarity to OsMYB from rice and LBMI from tobacco, showing 73% and 70% identity within the DNA-binding domains. However, the C-terminal domain of the SmP protein does not show obvious similarity to any other known protein sequence. It is rich in hydrophilic amino acids, especially in serine residues (18.38%), partly organized in homopolymeric stretches, a feature often found in activation domain of transcription factors.
