Evaluation of Amberlite XAD-1600 resin-based purification of the bioactive components from Radix Paeoniae Alba and Rhizoma Curcumae Longae extracts by pharmacodynamic and toxicological models.
- Author:
Zhi-hua WU
1
,
2
;
Zhong-qiu LIU
;
Yan-fang FANG
;
Jia-jie ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anti-Inflammatory Agents, Non-Steroidal; isolation & purification; pharmacology; Curcuma; chemistry; Drugs, Chinese Herbal; isolation & purification; pharmacology; toxicity; Female; Male; Mice; Mice, Inbred ICR; Paeonia; chemistry; Rats; Rats, Sprague-Dawley; Resins, Synthetic; chemistry
- From: Journal of Southern Medical University 2009;29(5):833-836
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the pharmacodynamics and toxicicity of the major bioactive components extracted and purified from Radix Paeoniae Alba and Rhizoma Curcumae Longae using Amberlite XAD-1600 resin.
METHODSAmberlite XAD-1600 was used to purify the bioactive components from the crude 75% ethanol extracts of the two herbs. The pharmacodynamic and toxic effects of the crude extracts and extract purified using XAD-1600 resin were comparatively examined with two acute inflammatory models, two pain models and acute toxicity test in vivo.
RESULTSThe anti-inflammatory and analgesic effects of the purified extract were significant stronger with lower toxicity than those of the crude ethanol extract.
CONCLUSIONAmberlite XAD-1600 resin allows efficient extraction and purification of the bioactive components from the two herbs.
