The significance of TGF-beta expression in scar in adenosine receptor A(2A) knockout mice.
- Author:
Hu XIAO
1
;
Li RAN
;
Ying-ying ZHUO
;
De-chang WANG
;
Ran HUO
;
Yi-bing WANG
;
Yong-qiang FENG
;
Qiang LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cicatrix; metabolism; pathology; Disease Models, Animal; Mice; Mice, Knockout; Receptor, Adenosine A2A; genetics; Transforming Growth Factor beta; genetics; metabolism
- From: Chinese Journal of Plastic Surgery 2010;26(2):136-138
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo discuss the mechanism of scar hypertrophy in adenosine receptor A(2A) (A(2A) R) knockout mice.
METHODSAnimal models of hypertrophic scar were established in 12 A(2A) R knockout mice and 12 wild-type mice as control. The thickness and the size of transverse section of the hypertrophic scar were observed by H-E staining. The hydroxyproline (HYP) in the scar was measured colorimetrically. The TGF-beta expression was tested by Western blotting method.
RESULTSThe hypertrophic scar in wild-type mice was more severe than that in knockout mice. Compared with self-control, the increase of the thickness and the size of transverse section of hypertrophic scar was markedly higher in wild-type group than in the knockout group (P < 0.01). There was significant difference in HYP content between the two groups (P < 0.01). Compared with self-control, the increase of TGF-beta expression in wild-type group was much more than that in knockout group (P < 0.01).
CONCLUSIONSThe TGF-beta expression decreases in the A(2A) R knockout mice. The scar hypertrophy is also much less in the A(2A) R knockout mice.
