Feasibility of cartilage link protein of hyaluronic acid for defining radiotherapeutic target volume in a mouse model of lung tumor.
- Author:
Zhi-Xin LIANG
1
;
Yong-Gang QIANG
;
Yong-Hua LIAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Autoradiography; methods; Extracellular Matrix Proteins; metabolism; pharmacology; Female; Hyaluronic Acid; metabolism; Immunohistochemistry; Iodine Radioisotopes; Lung Neoplasms; radiotherapy; Male; Mice; Proteoglycans; metabolism; pharmacology; Radiotherapy, Image-Guided; methods
- From: Journal of Southern Medical University 2012;32(3):301-305
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the feasibility of using cartilage link protein of hyaluronic acid (HA-CLP) for defining the tumor boundary in a mouse model of lung carcinoma.
METHODSLung carcinoma was induced in KM mice by chemical carcinogenesis. HA-CLP separated from bovine cartilage and purified by affinity chromatography was labeled with (125)I for autoradiography. Immunohistochemical analysis and Western blotting were used to examine the efficiency of HA-CLP in defining the boundaries of the lung tumors.
RESULTSWith autoradiography, the clearest image of lung cancer was obtained at 2 h. With immunohistochemical method, the tumor boundary was the most clearly displayed at 2 h when the strongest signals of HA-CLP was detected; Western blotting also showed the clearest bands of HA-CLP at 2 h.
CONCLUSIONHA-CLP has the immunogenicity of HABP, and can efficiently indicate lung tumor boundary in autoradiography and immunohistochemistry.
