Effects of interleukin-1 receptor antagonist on the apoptosis of eosinophil in guinea pig with asthma.
- Author:
Feng XU
1
;
Hong-quan ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Asthma; blood; chemically induced; pathology; Blood Proteins; metabolism; Bronchoalveolar Lavage Fluid; chemistry; Eosinophil Granule Proteins; Eosinophils; drug effects; pathology; Female; Guinea Pigs; Interleukin 1 Receptor Antagonist Protein; Interleukin-5; blood; Leukocyte Count; Male; Ovalbumin; Random Allocation; Receptors, Interleukin-1; antagonists & inhibitors; Ribonucleases; blood; metabolism; Sialoglycoproteins; pharmacology
- From: Acta Pharmaceutica Sinica 2003;38(9):661-664
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo evaluate the effect of interleukin-1 receptor antagonist(IL-1ra) on apoptosis and associated mechanism of eosinophil in guinea pig with asthma.
METHODSA model of guinea pig with asthma was established. After inhalation of different concentrations of IL-1ra, asthma was induced in the guinea pig for 8 days, the concentration of eosinophil cationic protein (ECP) in serum and bronchoalveolar lavage fluid (BALF), IL-5 in serum, the eosinophil counts and apoptosis were assayed by radioimmunology, enzyme-linked immunosorbent assay(ELISA), fluoromicroscope and light microscope.
RESULTSIL-1ra indirectly decreased the level of IL-5 in serum, improved the apoptosis of eosinophil(EOS) in lung, then decreased the level of ECP in serum and BALF.
CONCLUSIONInhalation of nebulized IL-1ra showed protective effect against asthma through change of the activity and infiltration of EOS in lung.
