Labeling and Biodistribution of Re-188-DTPA ( Diethylenetriaminepentaacetic acid ).
- Author:
Jin LEE
;
Dong Soo LEE
;
Young Ju KIM
;
Young Soo CHANG
;
Jae Min JEONG
;
Seung Ae SHIN
;
June Key CHUNG
;
Myung Chul LEE
;
Chang Soon KOH
- Publication Type:In Vitro ; Original Article
- Keywords:
Re-188-DTFA;
Labeling efficiency;
Biodistribution;
Radiotherapy
- MeSH:
Angioplasty;
Animals;
Baths;
Dogs;
Half-Life;
Hydrogen-Ion Concentration;
Kidney;
Mice;
Pentetic Acid;
Radiotherapy;
Rats;
Sodium Acetate;
Water
- From:Korean Journal of Nuclear Medicine
1997;31(4):427-432
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Re-188 is useful candidate for therapeutic radionuclide because it has a physical half life of 17 hours, contains beta ernissions suitable for therapy(maximum energy 2.12MeV) and emits a garnma ray that is suitable for quantitative diagnostic scanning(155keV). To use He-188 as a radionuclide compound of angioplasty balloon radiotherapy, we investigated the labelling method and biodistribution of Re-188- DTPA. We postulated that labeled Re-188-DTPA is preferable because it would be excreted via urinary system more easily than other compounds. To label Re-188 with DTI'A, 1ml of 222MBqI(6mCi) of Re-188 was added to DTPA solution(DTPA 20mg, SnC4 2HsO 10mg, pH 3.5) and boiled at 100C for 120min in water bath. pH was adjuted to 5 with 2.3Fo sodium acetate. I.abeling efficiency was measured using TLC-SG(acetone, saline). We evaluated biodistribution of Re-188-DTPA in sacrificed mice at 10 and 60 minutes after injection. We acquired images of kidneys, and drew tirne-activity r.urves in normal dogs and rats and calculated Trnax and Tl/2 in rats. The labelling efficiency was 95.7Yo on average. Labelling of Re-188-DTPA was stable(90% after 5hours) in vitro at room temperature. According to time-activity curves of dogs and rats, it took 15 to 20 minutes after injection for Re-188-DTPA to be washed out through kidneys. In conclusion, Re-188-DTPA was successfully labeled, Re-188-DTPA was stable in vitro and was excreted early via kidneys in animals. We could recornmend Re-188-DTPA as radionuclide of potential use in angioplasty balloon radiotherapy.
