Inhibitory Effect of Dexamethasone on TGF-β1 Expression of Rabbit Ciliary Pigment Epithelia Cultured in Vitro
- Author:
Xinchun XIONG
1
;
Zulian XI
;
Juan MIAO
;
Qinglin LI
;
Haijiang ZHANG
;
Yizhen HU
;
Houren WEI
Author Information
1. 华中科技大学同济医学院附属协和医院
- Keywords:
ciliary pigment epithelium;
transforming growth factor-beta1;
dexamethasone
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2006;26(3):369-371
- CountryChina
- Language:Chinese
-
Abstract:
In order to elucidate the effect of dexamethasone on the expression of transforming growth factor-beta1 (TGF-β1) in ciliary pigment epithelial (CPE) cells cultured in vitro, rabbit CPE cells were cultured in vitro, treated with DMEM medium containing 0,1×10-8 , 5×10-8 , 10 × 10-8 and 50×10-8 mol/L dexamethasone respectively for 5 days. The TGF-β1 expression was detected by immunohistochemistry Supervision methods and analyzed semi-quantitatively by HMIAS-2000 image system. As opposed to in vivo, rabbit CPE cells expressed TGF-β1 under cultured circumstance in vitro. The gray scales of the positive yellow staining in the groups of 1×10-8, 5×10-8, 10×10-8 and 50× 10-8 mol/L dexamethasone were 136. 57±4.43, 140. 20±6.10, 142.98±2. 99, 146. 80± 1.68 and 150. 05± 1.94 respectively. When the concentrations of dexamethasone were equal to or higher than 5×10-8 mol/L and, the expression of TGF-β1 was inhibited. 10-7 mol/L dexamethasone showed a significant inhibition. It was suggested that CPE cells possess the potential ability of synthesizing and expressing TGF-β1. The inhibition of TGF-β1 expression by dexamethasone may be beneficial to the treatment of proliferative vitroretinopathy, also exert some influence on the secretion of aqueous humor and ciliary inflammation.
