Effect of tetrandrine on bax, bcl-2 and TGF-β2 mRNA expressions in cultured human Tenon's capsule fibroblasts.
- Author:
Dai LI
1
;
Ming SUN
;
Qingchun LI
;
Fang YANG
;
Shaochun LIN
;
Kaili WU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Benzylisoquinolines; pharmacology; Cell Proliferation; drug effects; Cells, Cultured; Cicatrix; metabolism; prevention & control; Fibroblasts; cytology; drug effects; metabolism; Humans; Proto-Oncogene Proteins c-bcl-2; genetics; metabolism; RNA, Messenger; genetics; metabolism; Tenon Capsule; cytology; Transforming Growth Factor beta2; genetics; metabolism; bcl-2-Associated X Protein; genetics; metabolism
- From: Journal of Southern Medical University 2012;32(1):97-100
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of tetrandrine (Tet) on the expression of bax, bcl-2, and transforming growth factor-β2 (TGF-β2) mRNA in cultured human fibroblasts of Tenon's capsules (TCFS) and explore its possible mechanism.
METHODSThe third passage of TCFS cultured in vitro were exposed to 1×10(-5) mol/L Tet for 24 h, and real-time fluorescence quantitative PCR was used to detect the changes in the expressions of bax, bcl-2, and TGF-β2 mRNA.
RESULTSThe expression level of bax mRNA was obviously higher, while bcl-2 and TGF-β2 mRNA levels were significantly lower in Tet-treated TCFS than those in the control cells (P<0.05).
CONCLUSIONTet can inhibit the proliferation of TCFS possibly by reducing the expressions of bcl-2 and TGF-β2 mRNA, enhancing the expression of bax mRNA and inducing cell apoptosis, suggesting its potential in preventing fibrous scar formation after glaucoma filtration surgery.
