Long term in-vitro expansion reduces immune modulation function of placental chorionic villi mesenchymal stem cells.
10.7534/j.issn.1009-2137.2013.06.035
- Author:
Zhou-Xin YANG
1
;
Yue-Ru JI
1
;
Zhi-Bo HAN
2
;
You-Wei WANG
2
;
Lei MENG
2
;
Zhong-Chao HAN
2
;
Author Information
1. State Key Laboratory of Experimental Hematology, Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
2. State Key Laboratory of Experimental Hematology, Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China
- Publication Type:Journal Article
- MeSH:
Cells, Cultured;
Chorionic Villi;
immunology;
Female;
Humans;
Integrin alpha4;
metabolism;
Mesenchymal Stromal Cells;
cytology;
immunology;
Monocytes;
cytology;
Placenta;
cytology;
Pregnancy
- From:
Journal of Experimental Hematology
2013;21(6):1552-1556
- CountryChina
- Language:Chinese
-
Abstract:
The main aim of this study was to investigate the biological activities and immune modulation changes of chorionic villi mesenchymal stem cells (CV-MSC) after long term culture. The morphology of the CV-MSC of passage 3 and passage 9 were observed by microscopy, and their phenotypes were detected by flow cytometry. CV-MSC of passage 3 and 9 were co-cultured with PHA-stimulated PBMNC, and IFN-γ concentration in culture medium was detected by ELISA. The mRNA expression of COX-2, HGF and HLA-G in CV-MSC were detected by real-time PCR. The results showed that after long term culture, the CV-MSC kept the MSC morphology and most of the phenotypes including CD31, CD34, CD44, CD45, CD62L, CD73, CD90, CD105, CD117, CD151, CD235a, CD271 and HLA-DR, while the CD49d was significantly up-regulated. Immune modulation ability of CV-MSC was reduced and the mRNA expression of COX-2 and HGF was down regulated after long term culture, but the expression of HLA-G did not found to be obvious change. It is concluded that the long term in vitro expansion changes the expression of CD49d and reduces immune modulation of CV-MSC.
