Grape Seed Proanthocyanidin Extract Alleviates Arsenic-induced Oxidative Reproductive Toxicity in Male Mice.
- Author:
Shu Gang LI
1
;
Yu Song DING
1
;
Qiang NIU
1
;
Shang Zhi XU
1
;
Li Juan PANG
1
;
Ru Lin MA
1
;
Ming Xia JING
1
;
Gang Ling FENG
1
;
Jia Ming LIU
1
;
Shu Xia GUO
1
Author Information
- Publication Type:Journal Article
- Keywords: Arsenic; Grape seed proanthocyanidin extract; Nrf2 signaling; Reproductive toxicity
- MeSH: Animals; Antioxidants; metabolism; Arsenic; toxicity; Grape Seed Extract; pharmacology; Lipid Peroxidation; drug effects; Male; Mice; NF-E2-Related Factor 2; genetics; metabolism; Oxidative Stress; drug effects; Proanthocyanidins; pharmacology; Signal Transduction; drug effects; Sperm Count; Testis; cytology; drug effects; metabolism
- From: Biomedical and Environmental Sciences 2015;28(4):272-280
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo determine the ability of grape seed proanthocyanidin extract (GSPE) in alleviating arsenic-induced reproductive toxicity.
METHODSSixty male Kunming mice received the following treatments by gavage: normal saline solution (control); arsenic trioxide (ATO; 4 mg/kg); GSPE (400 mg/kg); ATO+GSPE (100 mg/kg); ATO+GSPE (200 mg/kg) and ATO+GSPE (400 mg/kg). Thereafter, the mice were sacrificed and weighed, and the testis was examined for pathological changes. Nuclear factor (erythroid-derived 2)-like 2 (Nrf2), heme oxygenase 1 (HO1), glutathione S-transferase (GST), NAD(P)H dehydrogenase, and quinone 1 (NQO1) expression in the testis was detected by real-time PCR. Superoxide dismutase (SOD), glutathione (GSH), total antioxidative capability (T-AOC), malondialdehyde (MDA), 8-hydroxydeoxyguanosine (8-OHdG), and reproductive indexes were analyzed.
RESULTSATO-treated mice showed a significantly decreased sperm count and testis somatic index and activity levels of SOD, GSH, and T-AOC than control group. Compared to the ATO-treated group, ATO +GSPE group showed recovery of the measured parameters. Mice treated with ATO+high-dose GSPE showed the highest level of mRNA expression of Nrf2, HO, NQO1, and GST.
CONCLUSIONGSPE alleviates oxidative stress damage in mouse testis by activating Nrf2 signaling, thus counteracting arsenic-induced reproductive toxicity.
