Expression of CD14 and Toll-like receptor 4 on Kupffer cells and its role in ischemia-reperfusion injury on rat liver graft.
- Author:
Yong PENG
1
;
Zuo-jin LIU
;
Jian-ping GONG
;
Hai-zhong LIU
;
Lin GAN
;
Shou-bai LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; In Vitro Techniques; Kupffer Cells; pathology; physiology; Lipopolysaccharide Receptors; genetics; metabolism; Liver Transplantation; pathology; physiology; Male; NF-kappa B; metabolism; RNA, Messenger; genetics; Rats; Rats, Wistar; Reperfusion Injury; pathology; physiopathology; Signal Transduction; Toll-Like Receptor 4; genetics; metabolism; Tumor Necrosis Factor-alpha; secretion
- From: Chinese Journal of Surgery 2005;43(5):274-276
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression of lipopolysaccharide (LPS) receptor CD14 and Toll-like receptor 4 (TLR4) on Kupffer cells and its role in ischemia-reperfusion injury (IRI) on rat liver graft.
METHODSThe Kupffer cells following IRI were isolated and divided into control, ischemia-reperfusion (IR), and anti-CD14 antibody group. The mRNA and protein expression of CD14 and TLR4, nuclear factor kappa B (NF-kappaB) activity and TNF-alpha level in supernatant were measured.
RESULTSThe mRNA and protein expression of CD14 and TLR4 in IR group were significantly higher than those in control group (P < 0.01). The NF-kappaB activity and TNF-alpha level in IR group were significantly higher than those in control group (P < 0.01), and they greatly decreased after anti CD14 antibody treatment (compared with IR group, P < 0.05), but were still significantly higher than those in control group (P < 0.01).
CONCLUSIONSLPS following IRI could up-regulate CD14 and TLR4 gene and protein expression on Kupffer cells, and subsequently activate NF-kappaB to produce cytokines, but other signal transduction pathways might also participate in the IRI.
