Exploration of optimum conditions for production of saccharogenic mixed grain beverages and assessment of anti-diabetic activity.
- Author:
Jae Sung LEE
1
;
Yun Hwan KANG
;
Kyoung Kon KIM
;
Yeong Kyeong YUN
;
Jun Gu LIM
;
Tae Woo KIM
;
Dae Jung KIM
;
Sang Yeon WON
;
Moo Hoan BAE
;
Han Seok CHOI
;
Myeon CHOE
Author Information
- Publication Type:Original Article
- Keywords: response surface methodology; volatile compounds; saccharogenic mixed grain beverage; alpha-glucosidase; anti-diabetic activity
- MeSH: alpha-Glucosidases; Aspergillus oryzae; Beverages*; Blotting, Western; Edible Grain*; Ethanol; Ether; Gelatin; Glucose; Palmitic Acid; Reverse Transcriptase Polymerase Chain Reaction
- From:Journal of Nutrition and Health 2014;47(1):12-22
- CountryRepublic of Korea
- Language:Korean
- Abstract: PURPOSE: This study was conducted to establish the production conditions through optimization of the production process of beverages using Aspergillus oryzae CF1001, and to analyze volatile compounds and antidiabetic activity. METHODS: The optimum condition was selected using the response surface methodology (RSM), through a regression analysis with the following independent variables gelatinization temperature (GT, X1), saccharogenic time (ST, X2), and dependent variable; DeltaE value (y). The condition with the lowest DeltaE value occurred with combined 45 min ST and 50degrees C GT. The volatile compounds were analyzed quantitatively by GC-MS. RESULTS: Assessment of antidiabetic activity of saccharogenic mixed grain beverage (SMGB) was determined by measurement of alpha-glucosidase inhibition activity, and glucose uptake activity and glucose metabolic protein expression by reverse transcriptase polymerase chain reaction (RT-PCR) and western blot analysis. Results of volatile compounds analysis, 62 kinds of volatile compounds were detected in SMGB. Palmitic acid (9.534% ratio), benzaldehyde (8.948% ratio), benzyl ethyl ether (8.792% ratio), ethyl alcohol (8.35% ratio), and 2-amyl furan (4.826% ratio) were abundant in SMGB. We confirmed that alpha-glucosidase inhibition activity, glucose uptake activity, and glucose-metabolic proteins were upregulated by SMGB treatment with concentration dependent manner. CONCLUSION: Saccharogenic mixed grain beverage (SMGB) showed potential antidiabetic activity. Further studies will be needed in order to improve the taste and functionality of SMGB.
