Immunophenotype and differential diagnosis of Hodgkin's lymphoma.
- Author:
Yanhui LIU
1
;
Hengguo ZHUANG
;
Xinbo LIAO
;
Xinlan LUO
;
Donglan LUO
;
Xiuling CAI
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, CD; analysis; Diagnosis, Differential; Hodgkin Disease; immunology; metabolism; pathology; Humans; Immunohistochemistry; Immunophenotyping; Membrane Proteins; analysis; Mucin-1; analysis; Poly(A)-Binding Proteins; Proteins; RNA-Binding Proteins; analysis; T-Cell Intracellular Antigen-1
- From: Chinese Journal of Hematology 2002;23(10):524-527
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the immunophenotype and differential diagnosis of Hodgkin's lymphoma (HL).
METHODFifty six cases originally diagnosed as HL were re-evaluated according to lymphoma classification of WHO 2000 on paraffin sections using SP immunohistochemistry.
RESULTSAmong the 56 cases, 47 met the WHO criteria for HL, 8 were NHL and 1 metastatic tumor. Of the 47 HL cases, 2 were nodular lymphocyte predominant HL (NLPHL), 43 classical Hodgkin's lymphoma (CHL) and 2 unclassified HL, and of the 8 cases reclassified as NHL, 6 were T-cell rich B-cell lymphoma (TCRBCL) and 2 anaplastic large cell lymphoma (ALCL). In NLPHL cases, L&H cells were CD(20)(+), CD(15)(-) and CD(30)(-); CD(57)(+) cells and small B-lymphocytes predominated the background infiltration. Diagnostic R-S cells and other tumor cells in 43 cases of CHL were positive for CD(30) (100%), CD(15) (81%) and CD(20) (7%). Six cases of TCRBCL were negative for CD(15) and CD(30). Two cases of ALCL were positive for CD(30), ALK-1 and CD(3), and negative for CD(15) and CD(20). The reactive inflammatory infiltration in CHL and TCRBCL was rich in TIA-1 positive cytotoxic lymphocytes, and CD(57)(+) cells were rarely encountered.
CONCLUSIONCombining the immunophenotype of tumor cells and background cells with morphologic criteria are more helpful for classification of HL, and discrimination between NLPHL, CHL and TCRBCL.
