Study on the feasibility of a cell-culture inoculation test in the detection and isolation of street rabies virus

VernacularTitle:细胞培养法检测和分离狂犬病街毒的可行性研究
Author: Xia WANG ¹ ; Ge-Lin XU ; Jie WU ; Jing LUO ; Jia-Hong ZHU ; Jian-Jun ZHOU ; Nan-Feng SHI ; Qing TANG ; Yong-Zhen ZHANG ; Jian-Rong TANG ; Tai-Cai WU ; He-Tian MING ; Rui-Hua SHEN ; Huan-Chun CHEN
Author Information

1. 湖北省武汉市生物制品研究所
Keywords: Street rabies virus; Cell-culture inoculation test; Detection
From: Chinese Journal of Epidemiology 2009;30(6):611-615
CountryChina
Language:Chinese
Abstract: Objective Feasibility of using MNA cell-culture inoculation test to detect and isolate the street rabies virus. Methods Using MNA cell-culture inoculation test, fluorescent antibody test (FAT) and sandwich ELISA with double-antibodies to detect 33 specimens of street rabies virus, 20 specimens of negative canine brains and 4 specimens of healthy mice brains. Results 33 specimens of street rabies virus were positive to the cell-culture inoculation test but the others were negative. The concordances of MNA cell-cultured inoculation test with FAT and sandwich ELISA with double-antibodies were both 100%. Conclusion MNA cell-culture inoculation test appeared to be both highly sensitive and specific in detecting the street rabies virus, and could be used in detection and isolation of the virus.