Changes of leptin, tumor necrosis factor-alpha, neuropeptide Y levels and their association with insulin resistance and insulin secretion function in normal glucose tolerant first-degree relatives of familial type 2 diabetic pedigrees.
- Author:
Yan REN
1
;
Xiujun LI
;
Haoming TIAN
;
Jinzhong LIANG
;
Xiangxun ZHANG
;
Honglin YU
;
Jianan WANG
Author Information
1. Division of Endocrinology and Metabolism, West China Hospital of Sichuan University, Chengdu 610041, China. renyan22@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Adult;
Case-Control Studies;
Diabetes Mellitus, Type 2;
blood;
genetics;
Female;
Glucose Tolerance Test;
Humans;
Insulin;
secretion;
Insulin Resistance;
Leptin;
blood;
Male;
Middle Aged;
Neuropeptide Y;
blood;
Pedigree;
Tumor Necrosis Factor-alpha;
blood
- From:
Journal of Biomedical Engineering
2010;27(6):1341-1345
- CountryChina
- Language:Chinese
-
Abstract:
This investigation was made in regard to the changes of plasma Leptin, Tumor Necrosis Factor-alpha (TNF-alpha) and Neuropeptide Y (NPY) levels and their association with insulin resistance and beta-cell secretion function in normal glucose tolerant first-degree relatives of familial type 2 diabetic pedigrees in Chengdu area. Levels of Leptin, TNF-alpha, NPY and lipids (TG, TC, HDL-C) were determined in 86 type 2 diabetic mellitus (DM) patients, 73 normal glucose tolerant (NGT) first-degree relatives in familial type 2 diabetic pedigrees and 65 normal controls (NC) from non-diabetic families. All of the subjects underwent 75 g oral glucose tolerance test (OGTT). Plasma glucose, immunoreactive insulin (IRI) and true insulin (TI) levels were also determined. Fasting glucose and TI levels were used to calculate homeostasis model assessment-insulin resistance (HOMA-IR) and HOMA-beta cell indexes. After being adjusted for age and body mass index (BMI), the levels of Leptin in DM and NGT first-degree relatives were all significantly higher than that in normal controls (P < 0.05). Type 2 diabetic patients showed significantly elevated TNF-alpha levels than did the normal controls (P < 0.05). Furthermore, diabetic subjects showed significantly higher HOMA-IR and lower HOMA-B levels, compared with those in NGT and NC groups (P < 0.05). No statistically significant difference was found in regard to NPY among three groups. NGT first-degree relatives showed significantly higher levels of TG, fasting IRI, OGTT-2h IRI and HOMA-IR than did the normal controls (P < 0.05). Leptin was positively correlated with age, BMI, waist, A1c, fasting and OGTT-2h glucose, OGTT-2h TI and TNF-alpha in all subjects, and was negatively correlated with HOMA-B in females. Leptin levels were significantly elevated in NGT first-degree relatives, which implied that genetic defects of Leptin may play a role in the development of familial type 2 diabetic pedigrees.
