Effect of lipopolysaccharides from Porphyromonas endodontalis on the expression of macrophage colony stimulating factor in mouse osteoblasts
10.3760/cma.j.issn.1002-0098.2014.09.006
- VernacularTitle:牙髓卟啉单胞菌脂多糖对小鼠成骨细胞巨噬细胞集落刺激因子表达的影响
- Author:
Yaqiong YU
1
;
Lihong QIU
;
Jiajie GUO
;
Liu QU
;
Liya XU
;
Ming ZHONG
Author Information
1. 中国医科大学口腔医学院牙体牙髓科·辽宁省口腔医学研究所
- Keywords:
Porphyromonas endodontalis;
Lipopolysaeeharides;
Osteoblasts;
Macrophage colony-stimulating factor;
NF-kappa B
- From:
Chinese Journal of Stomatology
2014;49(9):535-539
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of lipopolysaccharides(LPS) extracted from Porphyromonas endodontalis(Pe) on the expression of macrophage colony stimulating factor(M-CSF) mRNA and protein in MC3T3-E1 cells and the role of nucler factor-κB(NF-κB) in the process.Methods MC3T3-E1 cells were treated with different concentrations of Pe-LPS(0-50 mg/L) and 10 mg/L Pe-LPS for different hours (0-24 h).The expression of M-CSF mRNA and protein was detected by reverse transcription polymerase chain reaction(RT-PCR) and enzyme-linked immunoadsordent assay(ELISA).The cells untreated by Pe-LPS served as control.The expression of M-CSF mRNA and protein was also detected in 10 mg/L Pe-LPS treated MC3T3-E1 cells after pretreated with BAY 11-7082 for 1 h,a special NF-κB inhibitor.The groups were divided as follows,control group,BAY group(10 μmol/L BAY 11-7082 treated alone MC3T3-E1 cells),Pe-LPS group(10 mg/L Pe-LPS stimulated MC3T3-E1 cells for 6 h),BAY combine with Pe-LPS group (10 μmol/L BAY 11-7082 pretreated cells for 1 h and 10 mg/L of Pe-LPS stimulated MC3T3-E1 cells for 6 h).Results The level of M-CSF mRNA and protein increased significantly after treatment with different concentrations of Pe-LPS(0-50 mg/L),which indicated that Pe-LPS induced osteoblasts to express M-CSF mRNA and protein in dose dependent manners.The expression of M-CSF protein increased from(35±2) ng/L (control group) to (170±8) ng/L(50 mg/L group).Maximal induction of M-CSF mRNA expression was found in the MC3T3-E1 cells treated with 10 mg/L Pe-LPS for 6 h.After 6 h,the expression of M-CSF mRNA decreased gradually.The expression of M-CSF protein also increased with the treatment of 10 mg/L Pe-LPS for 10 h[(122±4) ng/L].After 10 h,the expression of M-CSF protein decreased gradually.The mRNA and proteins of M-CSF decreased significantly after pretreatment with 10 μmol/L BAY 11-7082 for 1 h.There was no significant difference between BAY group and the control.Conclusions Pe-LPS may induce the expression of M-CSF mRNA and protein in MC3T3-E 1 cells through the signaling of NF-κB.
