Simultaneous determination of plantainoside D and verbascoside from stem of Chirita longgangensis var. hongyao by RP-HPLC.
- Author:
Manyuan WANG
1
;
Yuanjie FAN
;
Jing ZHANG
;
Muxin GONG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Chromatography, Reverse-Phase; methods; Coumaric Acids; analysis; Disaccharides; analysis; Glucosides; analysis; Magnoliopsida; chemistry; Phenols; analysis; Plant Extracts; analysis; Plant Stems; chemistry
- From: China Journal of Chinese Materia Medica 2010;35(23):3188-3191
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a RP-HPLC method for simultaneous determination of phenylethanoid glycosides plantainoside D and verbascoside in Chirita longgangensis var. hongyao.
METHODThe analysis was performed on a Agilent C18 column (4.6 mm x 250 mm, 5 microm) with CH3CN-1% HAc (16:84)as mobile phase at a flow rate of 1.0 mL x min(-1), and at a column temperature of 30 degrees C. The detection wave length was 332 nm.
RESULTThe linear ranges of calibration of plantainoside D and verbascoside were 3.125-100.00 mg x L(-1) (r = 0.9998) and 25.00-500.0 mg x L(-1) (r = 0.9998). The average recoveries were 101.3% and 100.8% with RSD of 2.6% and 2.2% (n=9), respectively.
CONCLUSIONThe method is simple, accurate, reliable and can be used for the quality evaluation of C. longgangensis var. hongyao and its preparation.
