Characterizing effects of solvent specific morus alba components on rat platelet aggregation, vascular tension and macrophage nitrite production.
- Author:
Shuang LING
1
;
Hongping ZHANG
;
Dandan ZHANG
;
Lijun ZHANG
;
Ka BIAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aorta; drug effects; physiopathology; Cell Line; Drugs, Chinese Herbal; isolation & purification; pharmacology; In Vitro Techniques; Macrophages; drug effects; metabolism; Male; Mice; Morus; chemistry; Nitrites; metabolism; Platelet Aggregation; drug effects; Platelet Aggregation Inhibitors; isolation & purification; pharmacology; Rats; Rats, Sprague-Dawley; Vasodilation; drug effects; Vasodilator Agents; isolation & purification; pharmacology
- From: China Journal of Chinese Materia Medica 2010;35(22):3024-3028
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of different solvent extractions of Mori Ramulus on platelet aggregation, vascular tension, and nitrite production from macrophages stimulated by lipopolysaccharides and interferon-gamma.
METHODThe components of Mori Ramulus were extracted by EtoAc, n-BuOH and chloroform respectively. Platelet aggregation was induced by ADP, arachidonic acid and collagen in vitro; nitrite production of activated macrophages was measured by Griess assay, and the vasodilatory effects of three extractions were investigated by isometric tension changes of aortic rings.
RESULTChloroform extraction concentration-dependently inhibited platelet aggregation by arachidonic acid, and reduced vascular tension of PE preconstricted aorta rings with or without endothelium. On the other hand, extractions of EtoAc and n-BuOH demonstrated dose-dependent inhibition on macrophage NO production stimulated by LPS/IFN-gamma.
CONCLUSIONPharmacological activities of Mori Ramulus depend on solvent specific components. Chloroform extraction of Mori Ramulus may benefit cardiovascular diseases through its properties of anti-platelet aggregation and vasodilatation. The inhibition of macrophage activity by EtoAc and n-BuOH extractions suggested an anti-inflammation effect of the compound.
