Inhibition of Invasion and Up-regulation of E-cadherin Expression in Human Malignant Melanoma Cell Line A375 by (-)-Epigallocatechin-3-gallate
- Author:
WU YAN
1
;
LIN YUN
;
LIU HOUJUN
;
LI JIAWEN
Author Information
1. 华中科技大学同济医学院附属协和医院
- Keywords:
(-)-epigalloeatechin-3-gallate;
melanoma;
E-cadherin;
invasion
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2008;28(3):356-359
- CountryChina
- Language:Chinese
-
Abstract:
The inhibitory effects of (-)-epigallocatechin-3-gallate (EGCG) on the invasion of human malignant melanoma cell line A375 and the possible molecular mechanisms of this effect were investiaged. A375 cells were pretreated with 20μg/mL EGCG for 24,48 and 72h respectively and the E-cadherin expression was detected by Western blot analysis. A375 cells were also pretreated with different concentrations of EGCG (1,5,10 and 20μg/mL) for 72h and the expression of E-cadherin was measured by RT-PCR. The adhesion and invasion of A375 cells were tested by cell-matrigel adhesion assay and matrigel invasion assay respectively. The results showed that EGCG could significantly up-regulate the expression of E-cadherin time- and concentration-dependently (both P<0.05). Statistical analysis showed that A375 cells invasion was inhibited by EGCG and correlated with the up-regulation of E-cadherin expression. It was suggested that EGCG strongly inhibited invasion of A375 cells, and the inhibition mechanism was possibly associated with the up-regulation of E-cadherin expression.
