Study on in vitro biomineralization of enamel-binding peptide.
- Author:
Wei WEI
;
Zhou PENG
;
Jie DENG
;
Wei ZHANG
;
Jing MAO
- Publication Type:Journal Article
- MeSH:
Dental Enamel;
chemistry;
Peptides;
chemical synthesis;
chemistry;
Protein Binding;
Tooth Calcification
- From:
Journal of Biomedical Engineering
2014;31(1):132-135
- CountryChina
- Language:Chinese
-
Abstract:
We present the binding ability of a new peptide (CMPQVMPMC-) with dental enamel after being evaluated in the present study. Under a standard procedure, the recovery of M13 filamentous phage was greatly enhanced by displaying the peptide in phage coat protein p III. Then the cyclic peptide was synthesized using a solid method. The effect of the cyclic peptide in vitro biomineralization was tested in a single-diffusion microtiter plate gel system. Absorbance at 405 nm of each sample was recorded for 24 h at every 6 h intervals. The relatively increased values of each sample were expressed as percentages relative to the blank group (100%). The cyclic peptide resulted in a concentration-dependent delayed nucleation. In addition, the overall values of peptide groups at the end of 24 h were lower than those in the control group but much higher than those in the BSA control group.
