Copy number variations in pediatric acute myeloid leukemia.
- Author:
Jia-Yuan ZHANG
1
;
Li ZHANG
1
;
Min RUAN
1
;
Xiao-Ming LIU
1
;
Ye GUO
1
;
Wen-Yu YANG
1
;
Fang LIU
1
;
Tian-Feng LIU
1
;
Ben-Quan QI
1
;
Xiao-Fan ZHU
2
,
3
Author Information
- Publication Type:Journal Article
- MeSH: Child; DNA Copy Number Variations; Humans; Karyotype; Karyotyping; Leukemia, Myeloid, Acute; Multiplex Polymerase Chain Reaction; Prognosis
- From: Journal of Experimental Hematology 2015;23(2):295-299
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the copy number variations (CNV) of gene in pediatric acute myeloid leukemia (AML) and its correlation with clinical features and prognosis.
METHODSThe clinical data of 130 children aged <14 years with newly diagnosed AML from May 2006 to March 2013 were analyzed restrospectively. The CNV were analyzed by multiplex ligation-dependent probe amplification (MLPA). Thirty-eight normal children were selected in control group. All the data were statistically analyzed using SPSS16.0 software.
RESULTSgene CNV of 2p24.3(MYCN), 10q23(PTEN) and 13q14(RB1, MIR15A, DLEU) were detected in more than 10% of the patients. CNV were detected in 49 cases(37.7%). The median loss and gain CNV frequencies per sample were 4. The CNV of TP53 correlated significantly with relapse. The loss ond gain CNV have no influence to EFS, DSF and OS. CNV were detected in the twelve percent of patients, but they were not detected with routine karyotype method.
CONCLUSIONThe MLPA technique combined with karyotyping makes a substantial increase in the diagnostic rate. Patients with TP53 alterations have significantly higher relapse rate.
