Poly(ADP-ribosyl)ation of Apoptosis Antagonizing Transcription Factor Involved in Hydroquinone-Induced DNA Damage Response.

Xiao Xuan LING; Jia Xian LIU; Lin YUN; Yu Jun DU; Shao Qian CHEN; Jia Long CHEN; Huan Wen TANG; Lin Hua LIU

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Poly(ADP-ribosyl)ation of Apoptosis Antagonizing Transcription Factor Involved in Hydroquinone-Induced DNA Damage Response.

Author: Xiao Xuan LING ¹ ; Jia Xian LIU ² ; Lin YUN ² ; Yu Jun DU ³ ; Shao Qian CHEN ⁴ ; Jia Long CHEN ⁵ ; Huan Wen TANG ² ; Lin Hua LIU ²
Author Information

1. Department of Environmental and Occupational Health, Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan 523808, Guangdong, China; School of Public Health, Guangzhou Medical University, Guangzhou 510182, Guangdong, China.
2. Department of Environmental and Occupational Health, Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan 523808, Guangdong, China.
3. Electrocardiogram Department of Cardiovascular Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524023, Guangdong, China.
4. Department of Clinical Laboratory, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong, China.
5. Department of Occupational Health and Occupational Medicine, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, Guangdong, China.
Publication Type:Letter
MeSH: Antioxidants; toxicity; Apoptosis Regulatory Proteins; genetics; metabolism; Cell Line; DNA Damage; drug effects; Gene Expression Regulation; drug effects; Gene Silencing; Histones; genetics; metabolism; Humans; Hydroquinones; toxicity; Poly (ADP-Ribose) Polymerase-1; Poly(ADP-ribose) Polymerases; genetics; metabolism; Protein Transport; Repressor Proteins; genetics; metabolism
From: Biomedical and Environmental Sciences 2016;29(1):80-84
CountryChina
Language:English
Abstract: The molecular mechanism of DNA damage induced by hydroquinone (HQ) remains unclear. Poly(ADP-ribose) polymerase-1 (PARP-1) usually works as a DNA damage sensor, and hence, it is possible that PARP-1 is involved in the DNA damage response induced by HQ. In TK6 cells treated with HQ, PARP activity as well as the expression of apoptosis antagonizing transcription factor (AATF), PARP-1, and phosphorylated H2AX (γ-H2AX) were maximum at 0.5 h, 6 h, 3 h, and 3 h, respectively. To explore the detailed mechanisms underlying the prompt DNA repair reaction, the above indicators were investigated in PARP-1-silenced cells. PARP activity and expression of AATF and PARP-1 decreased to 36%, 32%, and 33%, respectively, in the cells; however, γ-H2AX expression increased to 265%. Co-immunoprecipitation (co-IP) assays were employed to determine whether PARP-1 and AATF formed protein complexes. The interaction between these proteins together with the results from IP assays and confocal microscopy indicated that poly(ADP-ribosyl)ation (PARylation) regulated AATF expression. In conclusion, PARP-1 was involved in the DNA damage repair induced by HQ via increasing the accumulation of AATF through PARylation.