Evaluation of Protective Effects of Bioactive Phytochemicals Against Methotrexate in Salmonella typhimurium TA1535/pSK1002 Coupled with Micronucleus Assay.
- Author:
Ying WU
1
;
Shao Bin GU
1
;
Hao LI
2
;
Jia Yi HE
3
;
Li LI
2
;
Jian Bo YANG
2
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antimetabolites, Antineoplastic; adverse effects; Cytoprotection; Drug Evaluation, Preclinical; Female; Male; Methotrexate; adverse effects; Mice; Micronucleus Tests; Phytotherapy; Plant Extracts; Random Allocation; Salmonella typhimurium
- From: Biomedical and Environmental Sciences 2016;29(2):148-152
- CountryChina
- Language:English
- Abstract: We evaluated the antimutagenic effects of 10 kinds of bioactive phytochemicals and some phytochemical combinations against methotrexate (MTX)-induced genotoxicity by the umu test in Salmonella typhimurium TA1535/pSK1002 combined with a micronucleus assay. We observed that allicin, proanthocyanidins, polyphenols, eleutherosides, and isoflavones had higher antimutagenic activities than the other five types of bioactive phytochemicals. At the highest dose tested, MTX-induced genotoxicity was inhibited by 25%-75%. Kunming mice treated by MTX along with bioactive phytochemical combinations showed significant reduction in micronucleus induction and sperm abnormality rate (P<0.01). These results indicate that bioactive phytochemical combinations can be potentially used as new cytoprotectors.
