Changes of bone morphogenetic protein-7 and inhibitory Smad expression in streptozotocin-induced diabetic nephropathy rat kidney.
- Author:
Qin YANG
1
;
Bing HAN
;
Ru-Jia XIE
;
Ming-Liang CHENG
Author Information
1. Department of Pathophysiology, Guiyang Medical College, Guiyang, China. qinyang@gmc.edu.cn
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Morphogenetic Protein 7;
genetics;
metabolism;
Collagen Type I;
metabolism;
Diabetes Mellitus, Experimental;
metabolism;
Diabetic Nephropathies;
metabolism;
Kidney;
metabolism;
pathology;
Male;
RNA, Messenger;
genetics;
metabolism;
Random Allocation;
Rats;
Rats, Wistar;
Smad6 Protein;
genetics;
metabolism;
Smad7 Protein;
genetics;
metabolism;
Transforming Growth Factor beta1;
metabolism
- From:
Acta Physiologica Sinica
2007;59(2):190-196
- CountryChina
- Language:Chinese
-
Abstract:
The present study was designed to observe the expressions of bone morphogenetic protein-7 (BMP-7) and inhibitory Smads in kidney of rats with diabetic nephropathy (DN), and explore the possible mechanism of DN. Male Wistar rats weighing 180-220 g were single injected with streptozocin (STZ, 55 mg/kg body weight) for 2, 4, 8 and 16 weeks to induce DN. Blood glucose, kidney weight/body weight and 24-hour urine protein in the control and DN rats were examined; the expressions of BMP-7, Smad6 and Smad7 were detected by using immunohistochemical techniques, Western blot and real-time PCR. The results showed that blood glucose and 24-hour urine protein in DN rats were higher than that in the control rats and kidney weight/body weight was also elevated in DN rats, especially in 16-week STZ-induced rats. The expressions of BMP-7 and Smad6 proteins in DN rats were elevated, while BMP-7 mRNA expression was increased 2 weeks after STZ injection and decreased 16 weeks after STZ injection. The expressions of Smad7 protein and mRNA were elevated in DN rats 2 weeks after STZ injection and decreased 16 weeks after STZ injection. In addition, the expressions of transforming growth factor-beta1 (TGF-beta1) and collagen type I (COL-I) mRNA were increased in DN rats. These results suggest in the early stage of DN, increase in BMP-7 and inhibitory Smad expression may play a role in the feedback regulation and restrain the development of DN.
