Correlation between Andrographis Herba preparation's fingerprint, pharmacological activity and preparation process.
- Author:
Ji-Ping LAN
1
;
Yan-Jun HU
1
;
Ya-Qi WANG
1
;
Xue-Cheng WANG
1
;
You XIONG
1
;
Ming YANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Andrographis Herba; Trichoderma koningiopsis; correlation; fingerprints; pharmacological activity; preparation process
- From: China Journal of Chinese Materia Medica 2016;41(15):2802-2808
- CountryChina
- Language:Chinese
- Abstract: Process design grants the quality connotation to products. This paper was to investigate the correlation between changes of chemical fingerprints of Andrographis Herba preparation and its pharmacological activity, and set up the bridge between key process and quality attributes. By referring to the preparation process of Andrographis Herba. preparation (extracting-concentrating-drying-granulation), HPLC fingerprints were employed to determine the difference of the effective materials of the intermediate micro components. Cluster analysis results indicated that the extraction link had great influence on quality connotation variation of Andrographis Herba preparation. The pharmacological activity of various intermediates was continuously decreased in the models of DPPH antioxidant activity and LPS-induced anti-inflammatory activity in mice peritoneal macrophages. Traditional high temperature treatment process was detrimental to its clinical effect from the curve equation between the key process parameters and pharmacodynamic activity. Partial least square (PLS) was used to construct spectrum-efficiency model equation, and it was verified that this equation could accurately predict the relationship between fingerprints and pharmacological activity, which would facilitate the subsequent evaluation of quality attributes and provide scientific basis for further quality control of the whole process.
