New tactics of human red blood cells stored at 4 degrees C-protective effect of antioxidant solution on red blood cells damage.
- Author:
En-Pu MA
1
;
Xiu-Zhen LIU
;
Ying HAN
;
Ming-Dong LIU
;
Su-Ping REN
;
An LIU
;
Peng JIN
;
Feng-Rong BU
;
Zu-Ze WU
Author Information
1. Institute of Blood Transfusion, Academy of Military Medical Sciences, Beijing 100850, China. enpuma@yahoo.com
- Publication Type:Journal Article
- MeSH:
Antioxidants;
pharmacology;
Blood Preservation;
methods;
Cold Temperature;
Erythrocyte Deformability;
drug effects;
Erythrocytes;
drug effects;
metabolism;
Free Radical Scavengers;
pharmacology;
Hemoglobins;
drug effects;
metabolism;
Humans;
Superoxide Dismutase;
pharmacology;
Time Factors
- From:
Journal of Experimental Hematology
2002;10(2):153-155
- CountryChina
- Language:Chinese
-
Abstract:
Aliquots of venous blood from healthy donor were collected in plastic blood storage bags with ACD, GMA or antioxidant solution (superoxide dismutase, SOD), respectively, and stored at 4 degrees C. After storage for varying periods, the parameters of the blood were detected in the blood samples. Results showed that the parameters of the blood stored at 4 degrees C for 75 days in SOD group were following: the recovery of RBC-Hb was 87.2%, plasma-Hb (mg/L) was 193.2, P50 (mmHg) was 34.0 (normal value was 33.1); deformability (DImax) was 0.2413 (74.3% of normal value). There was no evident hemolysis, color change, air bubble and clots. It was concluded that human RBC stored at 4 degrees C for 75 days with SOD solution, recovery of levels of RBC-Hb and plasma-Hb were accorded with the requirements of "Basic Demands of Blood Station" in China.
