Effects of tea polyphenols and tea pigments on cell cycle regulators in rat liver precancerous lesions.
- Author:
Xudong JIA
1
;
Chi HAN
;
Junshi CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blotting, Western; Cell Cycle Proteins; drug effects; genetics; metabolism; Cyclin D1; drug effects; metabolism; Cyclin-Dependent Kinase 4; Cyclin-Dependent Kinase Inhibitor p21; Cyclin-Dependent Kinases; genetics; Cyclins; drug effects; metabolism; Flavonoids; Intracellular Signaling Peptides and Proteins; Liver Neoplasms; genetics; metabolism; pathology; Male; Phenols; pharmacology; Pigments, Biological; pharmacology; Polymers; pharmacology; Polyphenols; Precancerous Conditions; genetics; metabolism; pathology; Proliferating Cell Nuclear Antigen; drug effects; metabolism; Proteins; Proto-Oncogene Proteins; RNA, Messenger; drug effects; genetics; metabolism; Rats; Rats, Wistar; Reverse Transcriptase Polymerase Chain Reaction; Tea; chemistry
- From: Chinese Journal of Preventive Medicine 2002;36(4):225-228
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESThis study is to investigate the effects of tea polyphenols and tea pigments on cell cycle regulators in rat liver precancerous lesions.
METHODSThe modified Solt-Farber precancerous liver rat model was used. Rats were given water, tea polypheol solution (0.1%) or tea pigment solution (0.1%) throughout the whole experiment (56 days). Cyclin D1, P21(WAF1/CIP1), GADD45 and PCNA protein expression were detected by Western blotting and the RT-PCR method was applied to study the expression of Cdk4.
RESULTSCyclin D1, Cdk4 and PCNA expressions were significantly inhibited, and the expression of P21(WAF1/CIP1) and GADD45 were significantly induced by tea polyphenols and tea pigments treatments.
CONCLUSIONTea polyphenols and tea pigments induced cell cycle arrest and inhibited cell proliferation by regulating cell cycle regulators.
