m-Nisodipine inhibited 5-HT-induced proliferation of rat PASMCs through Rho/ROCK signal pathway.
- Author:
Huan-long LIU
;
Ding YU
;
Zhong-ning ZHU
;
Su-wen SU
;
Xue-yan CHEN
;
Yong-jian ZHANG
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Proliferation;
drug effects;
Myocytes, Smooth Muscle;
cytology;
drug effects;
Nisoldipine;
pharmacology;
Protein Phosphatase 1;
metabolism;
Pulmonary Artery;
cytology;
Rats;
Serotonin;
pharmacology;
Signal Transduction;
rho-Associated Kinases;
metabolism;
rhoA GTP-Binding Protein;
metabolism
- From:
Acta Pharmaceutica Sinica
2015;50(7):824-829
- CountryChina
- Language:Chinese
-
Abstract:
This paper is to report the exploration of the activation of Rho/ROCK signal pathway in 5-HT-induced proliferation of rat pulmonary artery smooth muscle cells (PASMCs) and the inhibitory effect of m-Nis on this pathway. PASMCs were cultured with the explant technique. MTT assay was used to explore the proliferation of PASMCs after 5-HT treated for different time and the intervening effect of m-Nis. RT-PCR and Western blot were used respectively to explore the mRNA expression of RhoA, ROCK1 and the protein expression of p-MYPT1 in 5-HT-treated PASMCs and intervening effect of m-Nis. The results of MTT assay suggested that 5-HT (1 µmol · L(-1)) treatment for 12-72 h significantly induced the proliferation of rat PASMCs (P<0.05 or P < 0.01), which were inhibited by m-Nis (1 x 10(-5), 1 x 10(-6), l x 10(-7), 1 x10(-8) mol · L(-1)) in dose-dependent manners (P < 0.05 or P < 0.01). Similarly, the mRNA expression of RhoA, ROCK1 and the protein expression of p-MYPT1 were also inhibited by m-Nis in different degrees (P < 0.05 or P < 0.01). Thus, the results of this study suggested that Rho/ROCK pathway played an important role in 5-HT-induced proliferation of rat PASMCs, m-Nis inhibited 5-HT-induced proliferation obviously, which may be related to the blockage of Rho/ROCK signal pathway.
