Cloning and expression of three thaumatin-like protein genes from.
10.1016/j.apsb.2017.03.001
- Author:
Mengmeng LIU
1
;
Dawei ZHANG
1
;
Yongmei XING
1
;
Shunxing GUO
1
Author Information
1. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
- Publication Type:Journal Article
- Keywords:
Defense;
Expression;
Gene cloning;
Polyporus umbellatus;
Thaumatin-like protein
- From:
Acta Pharmaceutica Sinica B
2017;7(3):373-380
- CountryChina
- Language:English
-
Abstract:
Genes encoding thaumatin-like protein () are frequently found in fungal genomes. However, information ongenes inis still limited. In this study, threegenes were cloned from. The full-length coding sequence of,andwere 768, 759 and 561 bp long, respectively, encoding for 256, 253 and 187 amino acids. Phylogenetic trees showed that,andwere clustered with sequences fromand, respectively. The expression patterns of the threegenes were higher inwithinfection than in the sclerotia without. Furthermore, over-expression of three PuTLPs were carried out inBL21 (DE3) strain, and high quality proteins were obtained using Ni-NTA resin that can be used for preparation of specific antibodies. These results suggest that,andinmay be involved in the defense response toinfections.
