Rapid analysis on phenolic compounds in Rheum palmatum based on UPLC-Q-TOF/MSE combined with diagnostic ions filter.
- Author:
Qing WANG
1
;
Zhi-Wei LU
1
;
Yue-Hong LIU
1
;
Ming-Ling WANG
1
;
Shuang FU
1
;
Qing-Qing ZHANG
1
;
Hui-Zhen ZHAO
1
;
Zhi-Xin ZHANG
1
;
Zi-Ye XIE
1
;
Zheng-Hai HUANG
1
;
Hong-Hong YU
1
;
Wen-Juan ZHOU
1
;
Xiao-Yan GAO
1
Author Information
- Publication Type:Journal Article
- Keywords: Rheum palmatum; diagnostic ions; liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MSE); phenolic compounds
- From: China Journal of Chinese Materia Medica 2017;42(10):1922-1931
- CountryChina
- Language:Chinese
- Abstract: Diagnostic ions filter method was used to rapidly detect and identify the phenolic compounds in Rheum palmatum based on ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MSE). The representative authentic standards of phenolic compounds, including gallic acid, (+)-catechin, (－)-epicatechin, (－)-epicatechin-3-O-gallate and procyanidin B2, were subjected to analysis by UPLC-Q-TOF/MSE system with negative ion mode. Fragmentation patterns of each standard were summarized based on assigned fragment ions. The prominent product ions were selected as diagnostic ions. Subsequently, diagnostic ions filter was employed to rapidly recognize analogous skeletons. Combined with retention time, accurate mass, characteristic fragments and previous literature data, the structures of the filtered compounds were identified or tentatively characterized. A total 63 phenolic compounds (36 phenolic acid derivatives, 8 flavonoid derivatives and 19 tennis derivatives) in R. palmatum were identified, including 6 potential new compounds. The method of diagnostic ions filter could rapidly detect and identify phenolic compounds in R. palmatum This study provides a method for rapid detection of phenolic compounds in R. palmatum and is expected to complete the material basis of rhubarb.
