Quality evaluation of rhubarb dispensing granules based on multi-component simultaneous quantitative analysis and bioassay.
10.19540/j.cnki.cjcmm.2017.0118
- Author:
Peng TAN
1
;
Hai-Zhu ZHANG
2
;
Ding-Kun ZHANG
3
;
Shan-Na WU
3
;
Ming NIU
3
;
Jia-Bo WANG
3
;
Xiao-He XIAO
3
Author Information
1. Chengdu Institute for Food and Drug Control, Chengdu 610045, China.
2. Department of Pharmacy and Chemistry, Dali University, Dali 671000, China.
3. China Military Institute of Chinese Materia Medica, 302 Military Hospital, Beijing 100039, China.
- Publication Type:Journal Article
- Keywords:
biopotency;
correlation analysis;
multi-component simultaneous quantitative analysis;
quality evaluation;
rhubarb dispensing granules
- From:
China Journal of Chinese Materia Medica
2017;42(14):2683-2690
- CountryChina
- Language:Chinese
-
Abstract:
This study attempts to evaluate the quality of Chinese formula granules by combined use of multi-component simultaneous quantitative analysis and bioassay. The rhubarb dispensing granules were used as the model drug for demonstrative study. The ultra-high performance liquid chromatography (UPLC) method was adopted for simultaneously quantitative determination of the 10 anthraquinone derivatives (such as aloe emodin-8-O-β-D-glucoside) in rhubarb dispensing granules; purgative biopotency of different batches of rhubarb dispensing granules was determined based on compound diphenoxylate tablets-induced mouse constipation model; blood activating biopotency of different batches of rhubarb dispensing granules was determined based on in vitro rat antiplatelet aggregation model; SPSS 22.0 statistical software was used for correlation analysis between 10 anthraquinone derivatives and purgative biopotency, blood activating biopotency. The results of multi-components simultaneous quantitative analysisshowed that there was a great difference in chemical characterizationand certain differences inpurgative biopotency and blood activating biopotency among 10 batches of rhubarb dispensing granules. The correlation analysis showed that the intensity of purgative biopotency was significantly correlated with the content of conjugated anthraquinone glycosides (P<0.01), and the intensity of blood activating biopotency was significantly correlated with the content of free anthraquinone (P<0.01). In summary, the combined use of multi-component simultaneous quantitative analysis and bioassay can achieve objective quantification and more comprehensive reflection on overall quality difference among different batches of rhubarb dispensing granules.
