Preparation and evaluation of a time-resolved fluoroimmunoassay kit for CA50 determination.
- Author:
Jian-feng HANG
1
;
Ying-song WU
;
Wei-wen XU
;
Wei-hong YU
;
Ying HUANG
;
Ming LI
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Aged, 80 and over; Antigens, Tumor-Associated, Carbohydrate; blood; Female; Fluoroimmunoassay; instrumentation; methods; Humans; Male; Middle Aged; Radioimmunoassay; Reagent Kits, Diagnostic; standards; Reference Standards; Reproducibility of Results
- From: Chinese Journal of Oncology 2007;29(3):189-192
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo prepare a rapid and sensitive diagnostic kit for detection of CA50 based on time-resolved fluoroimmunoassay.
METHODSA sandwich-TRFIA diagmostic kit was developed using anti-CA50 monoclonal antibody and all parameters of the kit were evaluated.
RESULTSThe linear measurement range of the kit was (5 - 300) U/ml. The sensitivity was 0.2 U/ml. The intra- and inter-assay coefficients of variation were 4.3% - 8.2% and 7.7% - 11.2%, respectively. There was no cross-reaction with CEA, CA12-5, CA15-3 and AFP. The cross reactivity with CA19-9 was 0.7 U/ml. The correlation coefficient of detection results of 107 blood samples between this newly developed kit and commercially available CA50 RIA kit was 0.901.
CONCLUSIONThis newly developed CA50-TRFIA kit is a valuable test tool for clinical application with even better sensitivity, specificity and accuracy.
