The effect of As2O3 on induction of apoptosis and inhibition of telomerase activity in colon cancer LS-174T cells.
- Author:
Xi-Shan WANG
1
;
Gui-Yu WANG
;
Hai-Tao XU
;
Kuan WANG
;
Ming LIU
;
Song-Bin FU
;
Jing-Shu GENG
;
Qi-Fan ZHANG
;
Xin-Shu DONG
;
Jia-Hong ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Arsenicals; administration & dosage; pharmacology; Cell Cycle; drug effects; Cell Line, Tumor; Cell Survival; drug effects; Colonic Neoplasms; pathology; prevention & control; ultrastructure; Dose-Response Relationship, Drug; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Humans; Inhibitory Concentration 50; Mice; Mice, Inbred BALB C; Mice, Nude; Microscopy, Electron; Microscopy, Fluorescence; Oxides; administration & dosage; pharmacology; Polymerase Chain Reaction; methods; Random Allocation; Telomerase; antagonists & inhibitors; genetics; metabolism; Time Factors; Tumor Burden; drug effects; Xenograft Model Antitumor Assays
- From: Chinese Journal of Oncology 2007;29(6):415-418
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the impact of arsenic trioxide (As2O3) on human colorectal carcinoma LS-174T cells and their activity of telomerase.
METHODSLS-174T cells and xenograft model of nude mice were treated with As2O3. The inhibitory effect of As2O3 on survival of LS-174T cells was determined by MTT assay. Apoptosis was determined by electron microscopy and fluorescence microscopy. Cell cycle was assessed by flow cytometry. Telomerase activity in LS-174T cells was determined by PCR-ELISA kit.
RESULTSWith the increasing concentration of As2O3, the ratio of living cells to dead cells decreased significantly, and the IC50 value was 5.23 micromol/L. Apoptosis curve appeared after 24 h and cells turned to apoptosis in a time-dependent manner. As2O3 inhibited the telomerase activity in cell extraction, obviously in a concentration-dependent and time-dependent manner. Inhibitiory effect of As2O3 on xenograft model of nude mice was observed by tumor volume and weight measurement, showing a significant difference between As2O3 and control groups (P < 0.05).
CONCLUSIONBoth the experiments in vitro and in vivo showed an inhibitory effect of As2O3 on colonrectal cancer S-174T cell growth, probably by induction of apoptosis and inhibition of telomerase activity.
