Engineering Biomaterials for Feeder-Free Maintenance of Human Pluripotent Stem Cells.
- Author:
Kisuk YANG
1
;
Joan LEE
;
Seung Woo CHO
Author Information
1. Department of Biotechnology, Yonsei University, Seoul, Korea. seungwoocho@yonsei.ac.kr
- Publication Type:Review
- Keywords:
Human pluripotent stem cells;
Animal feeder cells;
Feeder-free systems;
Biomaterials
- MeSH:
Animal Feed;
Biocompatible Materials;
Embryonic Stem Cells;
Extracellular Matrix Proteins;
Feeder Cells;
Humans;
Induced Pluripotent Stem Cells;
Peptides;
Pluripotent Stem Cells;
Polymers;
Proteins
- From:International Journal of Stem Cells
2012;5(1):1-5
- CountryRepublic of Korea
- Language:English
-
Abstract:
Human pluripotent stem cells (hPSCs) are capable of differentiating into any type of somatic cell, a characteristic that imparts significant therapeutic potential. Human embryonic stem cells and induced pluripotent stem cells are types of hPSCs. Although hPSCs have high therapeutic potential, their clinical relevance is limited by the requirement for animal feeder layers, which maintain their pluripotency and self-renewal. hPSCs grown on animal feeder cells are at high risk for pathogen contamination and can be affected by the immunogenicity of the feeder layer. The presence of animal feeder cells also limits the scalability of hPSCs in culture because of the high cost of culturing and batch-to-batch variations. Therefore, development of feeder-free systems is imperative for robust, lower-cost, xeno-free, scalable culture of hPSCs. Biomaterials engineered with bioactive molecules such as adhesion proteins and extracellular matrix proteins, or synthetic materials such as peptides and polymers, may provide alternative substrates to animal feeder cells. This article reviews biomaterial-based, feeder-free systems for hPSC growth and maintenance, which provide clinically relevant alternatives to feeder cell systems.
