Neuroprotective effects of paeonol in a cell model of Parkinson disease.
- Author:
Hao WANG
1
;
Zhao-Ming GENG
2
;
Zhi-Wei HU
1
;
Shu-Yan WANG
3
;
Bing ZHAO
3
Author Information
- Publication Type:Journal Article
- MeSH: 1-Methyl-4-phenylpyridinium; Acetophenones; pharmacology; Animals; Apoptosis; Caspase 3; metabolism; Cell Survival; Down-Regulation; Fluoresceins; Neuroprotective Agents; pharmacology; PC12 Cells; Parkinson Disease; drug therapy; Proto-Oncogene Proteins c-bcl-2; metabolism; Rats; Reactive Oxygen Species; metabolism; bcl-2-Associated X Protein; metabolism
- From: Journal of Zhejiang University. Medical sciences 2015;44(1):30-36
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of paeonol on neuron cell model of Parkinson disease (PD).
METHODSThe cell model of Parkinson disease was induced by treatment of 1-Methyl-4-phenylpyridinium (MPP+) in PC12 cells, the PD model cells were treated with 1 μmol/L, 3 μmol/L or 9 μmol/L paeonol for 24h, respectively. Cell viability and LDH leakage were detected by MTT and lactate dehydrogenase (LDH) assay; the apoptosis of PC12 cells was assessed by Hoechst 33258 staining and flow cytometry; reactive oxygen species (ROS) production was detected by DCFH-DA method; and the ratio of Bax/Bcl-2 and activation of caspase-3 were determined by Western blotting.
RESULTSMPP+ treatment significantly reduced cell viability, increased LDH leakage, enhanced the proportion of apoptotic cells and ROS production. In addition, MPP+ treatment dramatically increased the Bax/Bcl-2 ratio, and the activation of caspase-3. Compared to PD model group, paeonol treatment significantly enhanced cell viability, decreased LDH leakage, inhibited the proportion of apoptotic cells and ROS production, reduced the Bax/Bcl-2 ratio and the activated caspase-3 protein.
CONCLUSIONPaeonol can prevent PC12 cells from apoptosis induced by MPP+, and the mechanism may be associated with the down-regulation of ROS production, Bax/Bcl-2 ratio and Caspase-3 activation.
