Effects of CGRP on LPS-induced MMP-9 secretion by alveolar macrophages.
- Author:
Yong-ping LIU
1
;
Cha-xiang GUAN
;
Hong-bo BAI
;
Min ZHANG
;
Yan-ru CUI
;
Hui-jun LIU
;
Chang-qing ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Female; Lipopolysaccharides; adverse effects; Macrophages, Alveolar; secretion; Male; Matrix Metalloproteinase 9; metabolism; Rats; Rats, Wistar; Receptors, Calcitonin Gene-Related Peptide; metabolism
- From: Chinese Journal of Applied Physiology 2007;23(2):211-215
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo explore the effects of calcitonin-gene-related peptide (CGRP) on LPS-induced MMP-9 secretion by alveolar macrophages (AM) in vitro.
METHODSThe supernatant of LPS-induced Wistar rat AM from different intervention groups were collected to measure the activity by gelatin zymography.
RESULTS(Only secreting a small amount of MMP-9 with unstimulated AM, LPS stimulated MMP-9 production in a concentration-dependent manner (p < 0.01). (2) The activity of MMP-9 in CGRP intervention groups at different levels were significantly lower than those in non-intervention group (p < 0.01). (3) The inhibiting effects of CGRP were diminished by H-7 and W-7, an antagonist of protein kinase C (PKC) and calmodulin (CaM) (p < 0.05).
CONCLUSIONThese data suggested that CGRP involved in the MMP-9 secretion by AM, partly, via PKC and CaM pathway.
