Effects of scorpion venom heat-resistant protein on kainic acid induced-damage of cultured primitive rat hippocampal neuropeptide Y-nergic neurons.
- Author:
Yan-Hui FENG
1
;
De-Qin YU
;
Yan PENG
;
Sheng-Ming YIN
;
Yi-Ping SUN
;
Xue-Fei WU
;
Wan-Qin ZHANG
;
Jie ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Death; Cells, Cultured; Hippocampus; cytology; metabolism; Kainic Acid; pharmacology; Male; Neurons; drug effects; metabolism; pathology; Neuropeptide Y; metabolism; Rats; Rats, Sprague-Dawley; Scorpion Venoms; pharmacology
- From: Chinese Journal of Applied Physiology 2007;23(3):315-318
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effects of scorpion venom heat-resistant protein (SVHRP) on kainic acid induced-damage of cultured primitive rat hippocampal neuropeptide Y-nergic neurons.
METHODSWe observed morphological changes, celluar vigor, NPY-immunoreactivity and NPY mRNA expression by means of Thionine staining, MTT assay, immunocytochemistry and RT-PCR, respectively, on the primitively cultured Sprague-Dawley rat hippocampal neuron treated with KA and SVHRP for 24 h.
RESULTSMTT assay and morphologic analysis showed that SVHRP markedly increased neuron survival-rate, and protected them from kA-induced damage. The expression of NPY-immunoreactivity and NPY mRNA in SVHRP group increased obviously compared with other groups.
CONCLUSIONSVHRP protected the primitively cultured hippocampal neurons from KA-induced neuroexcitotoxicity and promoted the expression of NPY.
