Effect of hypoxia on the expressions of intermedin/ adrenomedullin2 in plasma and the tissues of heart and lung in rats.
- Author:
Yong-Sheng GONG
1
;
Lii ZHANG
;
Yi-Min GUO
;
Liang-Gang HU
;
Sun-Zhong MAO
;
Xiao-Fang FAN
;
Ping HUANG
;
Lin HONG
Author Information
- Publication Type:Journal Article
- MeSH: Adrenomedullin; blood; genetics; metabolism; Animals; Hypertension, Pulmonary; etiology; metabolism; Hypoxia; complications; metabolism; Lung; metabolism; Male; Myocardium; metabolism; Neuropeptides; blood; genetics; metabolism; RNA, Messenger; genetics; metabolism; Random Allocation; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Applied Physiology 2009;25(1):8-11
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the effect and significances of two-week hypoxia on the expression of intermedin/adrenomedullin2 (IMD/ADM2) in plasma and the tissues of heart and lung in rats.
METHODSTwenty male SD rats were randomly divided into normal control group and hypoxia group. The concentrations of IMD/ADM2 and adrenomedullin (ADM) in plasma, right ventricle and lung tissue were measured by radioimmunoassay. RT-PCR was used to detect the mRNA levels of IMD/ADM2 and ADM in right ventricle and lung tissue.
RESULTS(1) The mean pulmonary arterial pressure (mPAP) and the weight ratio of right ventricle (RV) to left ventricle plus septum (LV + S) of hypoxia group were significantly higher than those of normal control group (P < 0.01). (2) The concentrations of IMD/ADM2 and ADM in plasma were significantly higher in hypoxia group, compared with normal control group (P < 0.01). (3) The concentration of ADM in right ventricle and lung tissue in hypoxia group was significantly higher than that in normal control group (P < 0.01), while there was no significant difference in IMD/ADM2 between the two groups. (4) The mRNA levels of IMD/ADM2 and ADM in right ventricle and lung tissues were significantly up-regulated in hypoxia group (P < 0.05).
CONCLUSIONThe expressions of IMD/ADM2 peptides and gene in plasma, right ventricular and pulmonary tissues are different in the early-middle pathological proceeding of pulmonary hypertension induced by two-week hypoxia in rats.
