The effects of protooncogene on oocyte maturation mediated by cytokines.
- Author:
Li-Ping ZHENG
1
;
Jing-Lei WANG
;
Yue-Hui ZHENG
;
Lei WU
;
Qiu-Xiang XIAO
;
Fang LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Cytokines; physiology; Epidermal Growth Factor; physiology; Female; Intercellular Signaling Peptides and Proteins; physiology; Maturation-Promoting Factor; genetics; metabolism; Mice; Mitogen-Activated Protein Kinases; metabolism; Oocytes; cytology; growth & development; physiology; Oogenesis; drug effects; physiology; Proto-Oncogene Proteins c-myb; genetics; metabolism; RNA, Messenger; genetics; metabolism; Receptor, ErbB-2; genetics; metabolism; Tumor Necrosis Factor-alpha; physiology
- From: Chinese Journal of Applied Physiology 2009;25(1):74-79
- CountryChina
- Language:Chinese
-
Abstract:
AIMThe mechanisms of cytokines in regulating oocyte maturation is still little known. The present study attempt to investigate whether the protooncogene of c-erbB2, c-myb are involved in introducing of cytokines to regulate oocyte maturation.
METHODSThis research used mouse GV stage oocyte culture model in vitro and RT-PCR, Western blotting method to explore the effect of EGF, TNFalpha, ET-1 and NO on oocyte maturation; to analyze the c-erbB2 mRNA and c-myb mRNA expression and the phosphorylation of MAPK and cyclinB1 expression in oocytes affected by above cytokines.
RESULTSEGF(10 microg/L) stimulated meiosis of oocytes significantly, the level of c-erbB2 mRNA, c-myb mRNA were increased, and promoted the phosphorylation of MAPK and cyclinB1 expression; TNFalpha (1 microg/L) and ET-1 ((10(-1) mol/L) had the results to EGF. Low dose of SNP (10(-5)mol/L) had no effect on oocyte maturation, but could significantly reverse the suppression of dbcAMP on oocyte maturation.
CONCLUSIONc-erbB2 and c-myb were involved in introducing of cytokines to regulate oocyte maturation, might be the middle link in connection of the cytokines with MAPK and MPF in regulation oocyte maturation.
