Construction of the first genetic linkage map of Salvia miltiorrhiza Bge. using SSR, SRAP and ISSR markers.
- Author:
Zong CHENG-KUN
;
Zhen-qiao SONG
;
Hai-mei CHEN
;
Chang LIU
;
Jian-hua WANG
;
Lin-lin GUO
;
Tian LIU
;
Yu-ling PAN
- Publication Type:Journal Article
- MeSH:
Chromosome Mapping;
Genetic Linkage;
Genetic Markers;
Microsatellite Repeats;
Polymorphism, Genetic;
Salvia miltiorrhiza;
genetics
- From:
Acta Pharmaceutica Sinica
2015;50(3):360-366
- CountryChina
- Language:Chinese
-
Abstract:
The first genetic linkage map of Salvia miltiorrhiza was constructed in 94 F1 individuals from an intraspecific cross by using simple sequence repeat (SSR), sequence-related amplified polymorphism (SRAP) and inter-simple sequence repeat (ISSR) markers. A total of 93 marker loci in the linkage map, consisting of 53 SSR, 38 SRAP and 2 ISSR locus were made up of eight linkage groups, covered a total length of 400.1 cm with an average distance of 4.3 cm per marker. The length of linkage groups varied from 3.3 -132 cm and each of them included 2-23 markers, separately. The result will provide important basis for QTL mapping, map-based cloning and association studies for commercially important traits in S. miltiorrhiza.
