Role of progesterone in TLR4-MyD88-dependent signaling pathway in pre-eclampsia.
10.1007/s11596-013-1188-6
- Author:
Ying ZHU
1
;
Min WU
1
;
Chao-Ying WU
1
;
Ge-Qing XIA
2
Author Information
1. Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
2. Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. xgq67@yahoo.com.cn.
- Publication Type:Journal Article
- MeSH:
Adult;
Blotting, Western;
Cells, Cultured;
Dose-Response Relationship, Drug;
Enzyme-Linked Immunosorbent Assay;
Female;
Gene Expression;
drug effects;
Humans;
Interleukin-6;
metabolism;
Leukocytes, Mononuclear;
drug effects;
metabolism;
Myeloid Differentiation Factor 88;
genetics;
metabolism;
NF-kappa B;
genetics;
metabolism;
Pre-Eclampsia;
blood;
genetics;
metabolism;
Pregnancy;
Progesterone;
pharmacology;
Reverse Transcriptase Polymerase Chain Reaction;
Signal Transduction;
drug effects;
genetics;
Toll-Like Receptor 4;
genetics;
metabolism;
Tumor Necrosis Factor-alpha;
metabolism;
Young Adult
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2013;33(5):730-734
- CountryChina
- Language:English
-
Abstract:
The role of progesterone in the Toll-like receptor 4 (TLR4)-MyD88-dependent signaling pathway in pre-eclampsia was studied. Peripheral blood mononuclear cells (PBMCs) from pre-eclampsia (PE) patients were subjected to primary culture, and stimulated with different concentrations of progesterone (0, 10(-8), 10(-6), and 10(-4) mol/L). The mRNA expression of TLR4, MyD88 and nuclear factor-kappaB (NF-κB) was detected by using real-time PCR. The Ikappa-B protein expression was detected by using Western blotting. The expression of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the supernatant was determined by using ELISA. With the concentrations of progesterone increasing, the mRNA expression levels of TLR4, MyD88 and NF-κB in 2(-ΔΔCT) value were significantly decreased, and the IkappaB protein expression levels were significantly increased. The TNF-α and IL-6 expression showed a downward trend when the progesterone concentration increased, and there were significant differences among all of the groups (P<0.05). It was suggested that progesterone can inhibit the TLR4-MyD88-dependent signaling pathway in PE significantly and benefit for the pregnancy.
