Study on bone mesenchymal stem cells transfected by polyethylene glycol/bone morphogenetic protein-2.

Author: Li-Feng DING ; Gang ZHENG ; Jun YANG ; Zhen-Dong ZHOU ; Jian-Jun LI
Publication Type:Journal Article
MeSH: Animals; Bone Diseases; genetics; therapy; Bone Morphogenetic Protein 2; chemistry; genetics; metabolism; Bone and Bones; cytology; Gene Expression Regulation; Humans; Mesenchymal Stromal Cells; cytology; metabolism; Nanoparticles; chemistry; Polyethylene Glycols; chemistry; RNA, Messenger; genetics; metabolism; Rabbits; Transfection; methods
From: China Journal of Orthopaedics and Traumatology 2014;27(1):48-53
CountryChina
Language:Chinese
Abstract:
OBJECTIVEPolyethylene glycol/bone morphogenetic protein-2 (PEG/BMP-2) nanoparticles were transfected into Rabbit bone mesenchymal stem cells (rBMSCs) and the expression of BMP-2 was detected.
METHODSDissociated rBMSCs were primarily cultured in vitro and BMP-2 gene was transfected into rBMSCs by PEG/BMP-2 nanoparticals and lipofectamine, respectively. The efficiency of transfection was detected by flow cytometry and the expression of BMP-2 was detected by Western Blot and real time RT-PCR.
RESULTSPEG/BMP-2 nanoparticals were successfully synthesized and transfected into rBMSCs. Compared with the lipofectamine transfection group, PEG/BMP-2 transfection group had higher efficiency and higher BMP-2 expression.
CONCLUSIONPEG/BMP-2 nanoparticals transfected rBMSCs highly expressed BMP-2,which provided novel strategies for the treatment of bone defect.