Development and validation of HPLC-UV-MS method for the control of four anti-diabetic drugs in suspected counterfeit products.
- Author:
Xiu-mei DAI
1
;
Ning AN
;
Jian-min WU
;
Hui-yi LI
;
Qi-ming ZHANG
Author Information
1. National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.
- Publication Type:Journal Article
- MeSH:
1-Deoxynojirimycin;
analogs & derivatives;
analysis;
Acarbose;
analysis;
Chromatography, High Pressure Liquid;
methods;
Chromatography, Reverse-Phase;
Glycoside Hydrolase Inhibitors;
Hypoglycemic Agents;
chemistry;
Inositol;
analogs & derivatives;
analysis;
Spectrometry, Mass, Electrospray Ionization;
methods;
Spectrophotometry, Ultraviolet;
alpha-Glucosidases;
analysis
- From:
Acta Pharmaceutica Sinica
2010;45(3):347-352
- CountryChina
- Language:English
-
Abstract:
An HPLC-UV method has been developed for the determination of valibose, miglitol, voglibose and acarbose, the four anti-diabetic drugs. The separation was accomplished successfully by using reversed phase chromatography (Prevail carbohydrate column, 250 mm x 4.6 mm, 5 microm) with a gradient acetonitrile-phosphate buffer solution (pH 8.0) at a wavelength of 210 nm. Furthermore, the method of a high-performance liquid chromatography coupled with ESI-MS in positive ionization mode has been established. These two methods were successfully applied to the assay and qualitative detection of four alpha-glucosidase inhibitors in the potential counterfeit anti-diabetic drugs.
